It was only a little over 65 years ago that we began to realize that life is information-based, with coded genetic information embedded in alphabet-like molecules. In the years that followed, biochemists also began to discover that the chemical reactions carried on by life were unlike the valence-based reactions we learned about in chemistry class, but were machine-like, with moving parts that function mechanically like rotors and engines.
Email Cell types, cell subtypes, cell sub-subtypes…how low can you go? In these cases, splitting away single cells can so disturb them that they give skewed information about their RNA contents. Alternatively, one can opt to analyze single nuclei instead of single cells, thereby avoiding harsh enzymes.
This shortcut to deeper transcriptomic analyses, however, can start to seem like the long way around. According to scientists based at the Broad Institute, single-nucleus RNA-Seq techniques often rely on flow cytometry and microfluidic technologies that cannot handle massively parallel operations.
The Broad scientists, however, propose a solution: DroNc-Seq, a single-cell expression profiling technique that merges sNuc-Seq, a single-nucleus RNA sequencing method, with microfluidics, allowing massively parallel measurement of gene expression in structurally complicated tissues.
To scale the method up to the level needed to efficiently study thousands of nuclei at a time, the Broad team turned to microfluidics. Drop-Seq, a single-cell RNA-Seq scRNAseq technique that encapsulates single cells together with DNA barcoded-beads in microdroplets to greatly accelerate expression profiling experiments and reduce cost.
Moreover, DroNc-seq readily identified rare cell types without the need for enrichment. DroNc-Seq's robustness and accuracy suggest it could be a valuable addition to the stable of technologies being used as part of the Human Cell Atlas and other scRNAseq-based efforts.
According to Dolomite Bio, the transcriptomes acquired with the new single-nucleus technology are very similar to those from whole cells, albeit with a lower total transcript capture rate and greater representation of nuclear RNA and pre-mRNA.A Japanese research group verified the presence of a protein modification that is a unique mark in human cell nucleus.
This protein modification, Histone H4 lysine 20 acetylation (H4K20ac), was only discovered in plant cells and its existence in mammalian cells has been indirectly proven. Discovery Education helps incorporate game-based learning into the classroom with puzzlemaker.
Create a new puzzle now! Cell structure and function - PPT. Presentation Summary: The nucleus is the control center of the cell.
The nucleus contains nearly all the cell’s DNA and with it the coded instructions for. Job Openings. At Nucleus, our purpose is to deliver happiness and connections every day by being awesome.
This is the company’s calling and if you believe this to be your calling too, we want you to consider a job with us. Nov 15, · The single-nucleus RNA-Seq chip—based on the company’s established scRNA-Seq chip—is designed to produce smaller droplets, allowing straightforward, low .
"In our research, we have found the 'lock and key' that allow HIV to enter a cell's nucleus. Once inside, the virus can begin to replicate itself, spreading almost unchecked throughout the body.